Simple, robust methods for high-throughput nanoliter-scale DNA sequencing

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Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.

We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 (n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = ...

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ژورنال

عنوان ژورنال: Genome Research

سال: 2005

ISSN: 1088-9051

DOI: 10.1101/gr.4221805